Seven-time champion Djokovic is chasing Roger Federer's record of eight titles at the All England Club and his 25th Grand Slam title in total.

Czech Vondrousova, who became the first unseeded woman to lift the title last year, earned a far less welcome accolade after a 6-4 6-2 defeat by Spain's Jessica Bouzas Maneiro.

Alcaraz, 21, beat Novak Djokovic to the title a year ago and has since taken his Grand Slam haul to three after winning the French Open earlier this month and despite being seeded three will be favourite to rack up number four.

Alcaraz, also the reigning Queen's champion, was beaten 7-6 (7/3), 6-3 by home favourite Jack Draper in the biggest win of the British number one's career.

Spanish tennis chiefs announced Wednesday that 22-time Grand Slam winner Nadal and newly crowned French Open champion Carlos Alcaraz would be part of the country's team at the Games next month.

"In Roland Garros, knowing all the Spanish players who have won this tournament and be able to put my name on that amazing list is something unbelievable," Alcaraz told reporters.

The world number one is just one match away from becoming the first woman to win the tournament in three straight years since Justine Henin in 2007 after a 6-2, 6-4 victory.

he injury, sustained in his fourth-round win over Francisco Cerundolo, ended the Serb's quest to win a record-extending 25th Grand Slam trophy and will result in him losing his top ranking to Italian Jannik Sinner on Monday.

Italy's Jasmine Paolini reached her first Grand Slam semi-final at the French Open on Wednesday after dumping out fourth seed Elena Rybakina 6-2, 4-6, 6-4.

"Due to a torn medial meniscus in his right knee (discovered during an MRI scan performed today), Novak Djokovic... has been forced to withdraw from the Roland Garros tournament," organisers said in a statement.

De Minaur had never got past the second round at the tournament before this year, winning just three of 10 matches.

The 24-time Grand Slam title winner made the last 16 by defeating Italy's Lorenzo Musetti in a bruising five-set epic which concluded at 3:07 am Sunday.

His four-hour 29-minute victory also allowed the 37-year-old world number one to equal Roger Federer's record of 369 Grand Slam match wins.

Wimbledon champion Alcaraz, who made the semi-finals at Roland Garros last year, scrambled to a 6-3, 6-4, 2-6, 6-2 second round victory over a player taking part in only his second Grand Slam main draw.

Djokovic, chasing a fourth title at Roland Garros and record 25th Grand Slam triumph, came through against 142nd-ranked French opponent Pierre-Hugues Herbert 6-4, 7-6 (7/3), 6-4.

They are six young Italians dressed as carrots who are at the French Open in a colourful attempt to inspire Sinner to victory.

"Of course, on paper it is not the best draw," Nadal said ahead of the match on Court Philippe Chatrier

"It feels really nice to be back and I'm just really grateful to be here in front of everybody," said Osaka.

Nadal, who has won the French Open 14 times and will celebrate his 38th birthday on June 3, faces world number four Alexander Zverev in the first round in Paris on Monday.

He said if Nadal was in the French Open draw, he could not be ruled out, despite his injury problems and growing competition from a younger generation.